Introduction


    Proteins with expanded polyglutamine (PolyQ) repeats are involved in human neurodegenerative diseases, via a gain-of-function mechanism of neuronal toxicity involving protein conformational changes that result in the formation and deposition of -sheet rich aggregates. Aggregation is dependent on context, specifically the properties of the host protein such as domain architecture and location of the repeat tract. In order to explore this relationship in greater detail, here we describe PolyQ 2.0, an updated database that provides a comprehensive knowledgebase for human polyQ proteins. Specifically, the database details domain context information, protein structural and functional annotation, single point mutation, predicted disordered regions, protein-protein interaction partners, metabolic/signaling pathways, post-translational modification sites and evolutionary information. Multiple database functionalities have also been added, including database search with multiple keywords, and new entry submission. Currently the database contains 9 reviewed disease-associated polyQ proteins, 105 reviewed non-disease polyQ proteins and 146 un-reviewed polyQ proteins. We envisage that this updated database will be a useful resource for functional and structural investigation of human polyQ proteins.

    If you find PolyQ 2.0 useful, please cite us:
   Chen Li, Jeremy Nagel, Steve Androulakis, Christopher J. Lupton, Jiangning Song and Ashley M. Buckle. PolyQ 2.0: an Improved Version of PolyQ, a Database of Human Polyglutamine Proteins. Database, 2016, DOI:10.1093/database/baw021.

Reminder:
    (1) Preferred browsers for different operating systems:
        a. Windows: IE, Firefox, Chrome (32 bit)
        b. Apple: Safari and Firefox
        c. Linux: Firefox and Chrome (32 bit)
    (2) In order to display the website properly, please make sure that your browser has enabled java applet plugin. Please click here for most updated Java software.
    (3) Please also ensure that the WebGL has been enabled for your specific browser. Please click here to see whether WebGL is working for your browser. If not, you can just follow the links to enable WebGL for your browser.

        a. By default, Google Chrome and IE support WebGL;
        b. If you are using Safari, please click here to configure your browser.
        c. If you are using Firefox, please click here to configure your browser.
    (4) If Java applet cannot run in your browser due to the 'Java Security Issue', please go to the Java setting panel and add our website 'http://lightning.med.monash.edu/' into the 'Exception Site List'. Click here for more detail.
    (5) The purpose of this database is to provide biological annotations for human polyQ proteins. Such biological annotations may not be complete and are subject to frequent updates. Therefore it is strongly recommended that one should seek for multiple sources/databases when examining the protein functions and structures.